{"id":1022,"date":"2020-02-01T17:12:17","date_gmt":"2020-02-01T08:12:17","guid":{"rendered":"\/text\/?page_id=1022"},"modified":"2020-03-22T20:43:46","modified_gmt":"2020-03-22T11:43:46","slug":"recombinant_dna-html","status":"publish","type":"page","link":"\/text\/?page_id=1022","title":{"rendered":"Recombinant_DNA.html"},"content":{"rendered":"\n<p><a name=\"Recombinant_DNA_and_Gene_Cloning\"><\/a><\/p>\n\n\n\n<h1 class=\"has-text-align-center wp-block-heading\">\u7d44\u307f\u63db\u3048\u578b DNA \u3068\u907a\u4f1d\u5b50\u30af\u30ed\u30fc\u30cb\u30f3\u30b0<\/h1>\n\n\n\n<hr class=\"wp-block-separator is-style-wide\"\/>\n\n\n\n<p style=\"text-align: right;\"><a href=\"\/text\/wp-content\/uploads\/2020\/03\/Kimball.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3582\" src=\"\/text\/wp-content\/uploads\/2020\/03\/Kimball.png\" alt=\"Kimball\" width=\"414\" height=\"30\"><\/a><br>25 April 2014 \u7248\u3092\u7ffb\u8a33\u4e00\u90e8\u6539\u5909<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"\"><tbody><tr><td><b>\u3053\u306e\u30da\u30fc\u30b8\u306e\u5185\u5bb9<\/b><\/td><\/tr><tr><td> <ul> <li><a title=\"Recombinant_DNA.html\" href=\"\/text\/?page_id=1022#An_Overview\">\u6982\u8981\u56f3<\/a><\/li> <li><a href=\"\/text\/?page_id=1022#Plasmids\">\u30d7\u30e9\u30b9\u30df\u30c9<\/a><\/li> <li><a href=\"\/text\/?page_id=1022#kanamycin\">\u4e3b\u306a\u4f8b<\/a> <ul> <li><a href=\"\/text\/?page_id=1022#pAMP\">pAMP<\/a><\/li> <li><a href=\"\/text\/?page_id=1022#pKAN\">pKAN<\/a><\/li> <li><a href=\"\/text\/?page_id=1022#Ligation_Possibilities\">\u3044\u308d\u3044\u308d\u306a\u65ad\u7247\u306e\u518d\u7d50\u5408<\/a><\/li> <\/ul> <\/li> <li><a href=\"\/text\/?page_id=1022#Transforming_E._coli\">\u5927\u8178\u83cc\u306e\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048<\/a><\/li> <li><a href=\"\/text\/?page_id=1022#Cloning_other_Genes\">\u907a\u4f1d\u5b50\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0<\/a><\/li> <li><a href=\"\/text\/?page_id=1022#Some_recombinant_DNA_products_being_used_in_human_therapy\">\u6cbb\u7642\u306e\u305f\u3081\u306e\u7d44\u307f\u63db\u3048 DNA \u7523\u7269<\/a><\/li> <\/ul> <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>\u7d44\u307f\u63db\u3048\uff08\u578b\uff09 DNA \uff08recombinant DNA\uff09 \u306f\u4eba\u5de5\u7684\u306b\u4f5c\u3089\u308c\u305f DNA \u3067\u3042\u308b\u3002 2 \u3064\u4ee5\u4e0a\u306e\u4f9b\u7d66\u6e90\u304b\u3089 DNA \u3092\u53d6\u308a\u51fa\u3057\uff0c 1 \u3064\u306e\u7d44\u307f\u63db\u3048\u5206\u5b50\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u3067\u5f62\u6210\u3055\u308c\u305f DNA \u5206\u5b50\u306e\u3053\u3068\u3067\u3042\u308b\u3002<\/p>\n\n\n\n<p><a name=\"Making_Recombinant_DNA_(rDNA):_An_Overview\"><\/a><\/p>\n\n\n\n<p><\/p>\n<a name=\"An_Overview\"><\/a>\n\n&nbsp;\n\n\n\n<h2 class=\"wp-block-heading\">\u7d44\u307f\u63db\u3048 recombinant DNA ( rDNA ) \u306e\u4f5c\u51fa : \u6982\u8981\u56f3<\/h2>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"\/text\/wp-content\/uploads\/2020\/02\/Making_rDNA.gif\" alt=\"\"\/><\/figure>\n\n\n\n<ul class=\"wp-block-list\"><li>2 \u3064\u306e\u4f9b\u7d66\u6e90\u304b\u3089\u53d6\u308a\u51fa\u3057\u305f DNA \u3092\u540c\u3058&nbsp;<a href=\"\/text\/?page_id=1050\">\u5236\u9650\u9175\u7d20<\/a>&nbsp;restriction endonuclease \u3067\u51e6\u7406\u3059\u308b ( \u3053\u306e\u4f8b\u3067\u306f BamHI \u3068\u3057\u3066\u793a\u3057\u3066\u3042\u308b ) \u3002<\/li><li>BamHI \u306f\u4e21\u65b9\u306e DNA \u5206\u5b50\u306e\u7279\u7570\u7684\u306a\u5869\u57fa\u914d\u5217\u3092\u8a8d\u8b58\u3057\u3066\uff0c\u3053\u308c\u3092 <strong>[G \/ GATCC] <\/strong> \u3068\u3044\u3046\u98a8\u306b\u5207\u65ad\u3059\u308b\u3002<br> <blockquote><p>5&#8242; GGATCC 3&#8242;<br>3&#8242; CCTAGG 5&#8242;<\/p><\/blockquote> <\/li><li>\u5207\u65ad\u3055\u308c\u305fDNA\u672b\u7aef\u306b\u306f 1 \u672c\u9396 DNA \u306e\u7a81\u51fa\u3057\u305f\u90e8\u5206\uff08\u7a81\u51fa\u672b\u7aef\uff09\u304c\u3042\u308b\u3002<\/li><li>\u3053\u308c\u3089\u306f\u76f8\u88dc\u7684\u306a\u7a81\u51fa\u672b\u7aef\u3092\u542b\u3080DNA \u5206\u5b50\u3068\u5869\u57fa\u5bfe\u5408\u3059\u308b\u306e\u3067\uff0c\u3053\u308c\u3089\u306f&nbsp;<b>\u4ed8\u7740\u672b\u7aef<\/b>&nbsp;sticky end \u3068\u547c\u3070\u308c\u308b\u3002<\/li><li>\u3053\u306e\u4f8b\u3067\u306f\uff0c\u4e21\u65b9\u306e DNA \u8a66\u6599\u304c\u76f8\u88dc\u7684\u306a\u4ed8\u7740\u672b\u7aef\u3092\u3082\u3064\u306e\u3067\uff0c\u6df7\u5408\u3055\u308c\u305f\u6642\u306b\u304a\u4e92\u3044\u306b\u5869\u57fa\u5bfe\u5408\u3059\u308b\u3002<\/li><li>DNA \u30ea\u30ac\u30fc\u30bc\u304c\u3053\u308c\u3089 2 \u3064\u3092\u5171\u6709\u7d50\u5408\u3055\u305b\uff0c 1 \u3064\u306e&nbsp;<b>\u7d44\u307f\u63db\u3048 DNA&nbsp;<\/b>\u5206\u5b50\u3068\u3059\u308b\u3002<\/li><\/ul>\n\n\n\n<p><a name=\"cloning\"><\/a><\/p>\n\n\n\n<p>\u5229\u7528\u3057\u3084\u3059\u3044\u3088\u3046\u306b, \u7d44\u307f\u63db\u3048\u5206\u5b50\u306f\u591a\u6570\u8907\u88fd\u3057\u3066\uff0c<a href=\"\/text\/?page_id=715\">\u5869\u57fa\u914d\u5217\u3092\u6c7a\u5b9a<\/a>&nbsp;\u306a\u3069\u306e\u5206\u6790\u306e\u8a66\u6599\u3068\u3059\u308b\u3002\u540c\u4e00\u7d44\u307f\u63db\u3048\u5206\u5b50\u306e\u30b3\u30d4\u30fc\u3092\u591a\u6570\u4f5c\u88fd\u3059\u308b\u3053\u3068\u3092&nbsp;<b>\u30af\u30ed\u30fc\u30cb\u30f3\u30b0<\/b>&nbsp;cloning \u3068\u547c\u3070\u308c\uff0c\u901a\u5e38&nbsp;<b>\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc<\/b>&nbsp;<b>p<\/b>olymerase&nbsp;<b>c<\/b>hain&nbsp;<b>r<\/b>eaction (&nbsp;<b>PCR<\/b>&nbsp;) \u3068\u3044\u3046\u6280\u8853\u304c\u7528\u3044\u3089\u308c\u308b\u3002\u3057\u304b\u3057\uff0c\u3053\u3053\u3067\u306f\u751f\u4f53\u5185\u3067\u3069\u306e\u3088\u3046\u306b\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u304c\u8d77\u3053\u3063\u3066\u3044\u308b\u304b\u3092\u307f\u3066\u307f\u3088\u3046\u3002<\/p>\n\n\n\n<p>\u4ee5\u4e0b\u306e\u7d30\u80de\u3067\u751f\u4f53\u5185\u3067\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u304c\u53ef\u80fd\u3067\u3042\u308b\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>\u5358\u7d30\u80de\u6027\u306e&nbsp;<a href=\"\/text\/?page_id=297#prokaryote_eukaryote\">\u524d\u6838\u7d30\u80de<\/a>&nbsp;(&nbsp;<b><a href=\"\/text\/?page_id=297#E_coli\">\u5927\u8178\u83cc<\/a><\/b>\u306a\u3069 \uff09<\/li><li>\u5358\u7d30\u80de\u6027\u306e&nbsp;<a href=\"\/text\/?page_id=297#prokaryote_eukaryote\">\u771f\u6838\u7d30\u80de<\/a>&nbsp;(&nbsp;<b>\u9175\u6bcd<\/b>\u306a\u3069 \uff09<\/li><li>\u7d44\u7e54\u57f9\u990a\u3067\u5897\u6b96\u3059\u308b\u54fa\u4e73\u52d5\u7269\u7d30\u80de<\/li><\/ul>\n\n\n\n<p>\u3059\u3079\u3066\u306e\u5834\u5408\u306b\u304a\u3044\u3066\uff0c \u8907\u88fd\u3055\u308c\uff0c\u767a\u73fe\u3055\u308c\u5f97\u308b\u5f62\u614b\u3067\u7d44\u307f\u63db\u3048 DNA \u304c\u7d30\u80de\u306b\u53d6\u308a\u8fbc\u307e\u308c\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u3002<a name=\"vector\"><\/a>\u3053\u308c\u3092\u5b9f\u73fe\u3059\u308b\u305f\u3081\u306b\uff0c<a href=\"\/text\/?page_id=297#Vector\"><b>\u30d9\u30af\u30bf\u30fc<\/b>&nbsp;vector<\/a> \u304c\u4f7f\u308f\u308c\uff0cDNA \u304c\u5c0e\u5165\u3055\u308c\u3066\u3044\u308b\u3002\u591a\u304f\u306e\u30a6\u30a4\u30eb\u30b9\u304c\u7d30\u83cc\u3084\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u306b\u5bfe\u3059\u308b\u30d9\u30af\u30bf\u30fc\u3068\u3057\u3066\u4f7f\u308f\u308c\u3066\u3044\u308b\u3002\u3053\u3053\u3067\u306f\uff0c\u5927\u8178\u83cc\u3092\u5bbf\u4e3b\u3068\u3057\u3066\uff0c<b>\u30d7\u30e9\u30b9\u30df\u30c9<\/b>&nbsp;plasmid \u3092\u30d9\u30af\u30bf\u30fc\u3068\u3057\u305f\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u4f8b\u3092\u793a\u3059\u3002<\/p>\n\n\n\n<p><a name=\"Plasmids\"><\/a><\/p>\n\n&nbsp;\n\n\n\n<h2 class=\"wp-block-heading\">\u30d7\u30e9\u30b9\u30df\u30c9 plasmid<\/h2>\n\n\n\n<p>\u30d7\u30e9\u30b9\u30df\u30c9\u306f\uff0c\u7d30\u83cc\u3067\u898b\u3064\u304b\u3063\u305f\u67d3\u8272\u4f53\u3068\u306f\u5225\u500b\u306e DNA \u5206\u5b50\u3067\u3042\u308b\u3002\u30d7\u30e9\u30b9\u30df\u30c9\u306f:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>\u975e\u5e38\u306b\u5c0f\u3055\u304f ( \u6570\u5343\u306e\u5869\u57fa\u3092\u542b\u3080 ) \uff0c<\/li><li>\u907a\u4f1d\u5b50\u3092 1 \u307e\u305f\u306f 2 &#8211; 3 \u500b\u3082\u3061\uff0c<\/li><li>\u74b0\u72b6 \u3067\uff0c<\/li><li>1 \u500b\u306e&nbsp;<b><a href=\"\/text\/?page_id=713#replication_origin\">\u8907\u88fd\u8d77\u70b9<\/a><\/b>&nbsp;\u3092\u3082\u3064\u3002<\/li><\/ul>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"290\" height=\"235\" src=\"\/text\/wp-content\/uploads\/2020\/03\/plasmids.png\" alt=\"\" class=\"wp-image-4168\"\/><figcaption>\u7d30\u80de\u5185\u306eDNA\u3092\u653e\u51fa\u3057\u305f\u5927\u8178\u83cc\u306e\u96fb\u5b50\u9855\u5fae\u93e1\u5199\u771f<br> \u7cf8\u72b6\u306e\u90e8\u5206\u306f\uff0c\u304a\u3088\u305d4,300\u306e\u907a\u4f1d\u5b50\u3092\u30b3\u30fc\u30c9\u3057\u3066\u3044\u308b460\u4e07\u4ee5\u4e0a\u306e\u5869\u57fa\u5bfe\u3092\u542b\u3093\u3067\u3044\u308b\u4e00\u672c\u9396DNA\u5206\u5b50\u3067\u3042\u308b\u3002\u5c0f\u3055\u306a\u8f2a\u72b6\u306e\u90e8\u5206\u306f\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u6307\u3059\u3002<br> \uff08\u30cf\u30fc\u30d0\u30fc\u30c9\u533b\u79d1\u5927\u5b66\u9662\u306e\u30cf\u30f3\u30c6\u30a3\u30f3\u30c8\u30f3\u30fb\u30dd\u30c3\u30bf\u30fc\u3068\u30c7\u30a4\u30d3\u30c3\u30c9\u30fb\u30c9\u30ec\u30b9\u30e9\u30fc\u306e\u5fa1\u597d\u610f\u306b\u3088\u308b\u3002\uff09<\/figcaption><\/figure><\/div>\n\n\n\n<p>\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u7d30\u83cc\u306e\u67d3\u8272\u4f53\u3068\u540c\u3058\u6a5f\u69cb\u3067\u8907\u88fd\u3055\u308c\u308b\u3002\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u306f\uff0c\u67d3\u8272\u4f53\u3068\u540c\u3058\u5272\u5408\u3067\u30b3\u30d4\u30fc\u3055\u308c\u3082\u306e\u3068 ( \u3053\u306e\u5834\u5408\u306f 1 \u500b\u306e\u7d30\u80de\u306b 1 \u30b3\u30d4\u30fc\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u542b\u307e\u308c\u308b ) \uff0c\u591a\u91cf\u306b\u30b3\u30d4\u30fc\u3055\u308c\u308b\u3082\u306e\u304c\u3042\u308b ( \u3053\u306e\u5834\u5408\u306f 50 \u4ee5\u4e0a\u306e\u30b3\u30d4\u30fc\u3092\u3082\u3064\u3053\u3068\u304c\u3042\u308b ) \u3002<\/p>\n\n\n\n<p>\u591a\u91cf\u306e\u30b3\u30d4\u30fc\u6570\u3092\u3082\u3064\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u907a\u4f1d\u5b50\u306f\u305f\u3044\u3066\u3044\u9ad8\u3044\u5272\u5408\u3067\u767a\u73fe\u3059\u308b\u3002\u81ea\u7136\u72b6\u614b\u3067\u306f\uff0c\u3053\u308c\u3089\u306e\u907a\u4f1d\u5b50\u306f&nbsp;<b>\u6297\u751f\u7269\u8cea<\/b>&nbsp;\u304b\u3089\u7d30\u83cc\u3092\u8b77\u308b\u30bf\u30f3\u30d1\u30af\u8cea ( \u9175\u7d20\u306a\u3069 ) \u3092\u30b3\u30fc\u30c9\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u591a\u3044\u3002<\/p>\n\n\n\n<p>\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u7d30\u83cc\u5185\u306b\u5bb9\u6613\u306b\u5165\u308a\u8fbc\u3080\u3002\u75c5\u9662\u5185\u306a\u3069\u3067\u6297\u751f\u7269\u8cea\u306b\u62b5\u6297\u6027\u3092\u3082\u3064\u83cc\u304c\u6025\u901f\u306b\u5e83\u304c\u3063\u305f\u308a\u3059\u308b\u306e\u306f\u3053\u306e\u305f\u3081\u3067\u3042\u308b\u3002\u3053\u306e\u6027\u8cea\u3092\u5229\u7528\u3057\u3066\uff0c\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u907a\u4f1d\u5b50\u5c0e\u5165\u306b\u7528\u3044\u3089\u308c\u308b\u3002<\/p>\n\n\n\n<p><a name=\"kanamycin\"><\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u4e3b\u306a\u4f8b<\/h2>\n\n\n\n<p>\u30b3\u30fc\u30eb\u30c9\u30b9\u30d7\u30ea\u30f3\u30b0\u30cf\u30fc\u30d0\u30fc\u7814\u7a76\u6240\u306e\u30c7\u30a4\u30d3\u30c3\u30c9\u30fb\u30df\u30af\u30ed\u30b9\u3068\u30b0\u30ec\u30c3\u30b0\u30fb\u30d5\u30e9\u30a4\u30a2\u30fc\u306e\u5fa1\u597d\u610f\u306b\u3088\u308b\u3002\u5f7c\u3089\u306f\uff0c\u751f\u7269\u5b66\u306b\u771f\u5263\u306b\u53d6\u308a\u7d44\u3093\u3067\u3044\u308b\u3059\u3079\u3066\u306e\u5b66\u751f\uff08\u9ad8\u6821\u751f\u3084\u5927\u5b66\u751f\uff09\u304c\u7d4c\u9a13\u3059\u3079\u304d\u5b9f\u9a13\u57fa\u790e\u6280\u8853\u3068\u3057\u3066\uff0c\u7d44\u307f\u63db\u3048\uff24\uff2e\uff21\u6280\u8853\u3078\u3053\u308c\u3089\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u7528\u3044\u3066\u3044\u308b\u3002<\/p>\n\n\n\n<p><p><a name=\"pAMP\"><\/a><\/p>\n<p><\/p>\n\n&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">pAMP<\/h3>\n\n\n\n<ul class=\"wp-block-list\"><li>4539 \u500b\u306e\u5869\u57fa\u5bfe<\/li><li>\u5358\u4e00\u8907\u88fd\u958b\u59cb\u70b9<\/li><li>\u6297\u751f\u7269\u8cea&nbsp;<b>\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3<\/b>&nbsp;ampicillin ( \u9178\u306b\u5b89\u5b9a\u3067\u7d4c\u53e3\u6295\u4e0e\u53ef\u80fd\u306a\u5e83\u7bc4\u56f2\u30da\u30cb\u30b7\u30ea\u30f3 ) \u8010\u6027\u306e\u907a\u4f1d\u5b50 (<em>&nbsp;<b>amp<sup>r<\/sup><\/b>&nbsp;<\/em>)<\/li><li>\u4ee5\u4e0b\u306e\u5869\u57fa\u306e<b>\u5358\u72ec<\/b>\u51fa\u73fe<br> <blockquote><p>5&#8242; GGATCC 3&#8242;<br>3&#8242; CCTAGG 5&#8242;<\/p><\/blockquote> <p>\u3053\u306e\u90e8\u5206\u306f\u4e0a\u8ff0\u306e\u3088\u3046\u306b&nbsp;<a href=\"\/text\/?page_id=1050\">\u5236\u9650\u9175\u7d20<\/a>&nbsp;<b>BamHI<\/b>&nbsp;\u3067\u5207\u65ad\u3067\u304d\u308b\u3002<\/p> <\/li><li>\u4ee5\u4e0b\u306e\u5869\u57fa\u306e<b>\u5358\u72ec<\/b>\u51fa\u73fe<br> <blockquote><p>5&#8242; AAGCTT 3&#8242;<br>3&#8242; TTCGAA 5&#8242;<\/p><\/blockquote> <p>\u3053\u306e\u90e8\u5206\u306f\u5236\u9650\u9175\u7d20&nbsp;<b>HindIII<\/b>&nbsp;\u3067\u5207\u65ad\u3067\u304d\u308b\u3002<\/p> <\/li><\/ul>\n\n\n\n<p>BamHI and HindIII \u306e\u4e21\u65b9\u3067 pAMP \u3092\u51e6\u7406\u3059\u308b\u3068\uff0c<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><b><em>amp<sup>r<\/sup><\/em><\/b>&nbsp;\u907a\u4f1d\u5b50\u3068\u8907\u88fd\u958b\u59cb\u70b9\u304c\u542b\u307e\u308c\u308b&nbsp;<b>3755<\/b>&nbsp;\u500b\u306e\u5869\u57fa\u5bfe\u306e\u65ad\u7247\u3068\uff0c<\/li><li><b>784<\/b>&nbsp;\u500b\u306e\u5869\u57fa\u5bfe\u306e\u65ad\u7247\u304c\u5f97\u3089\u308c\uff0c<\/li><li>\u4e21\u65ad\u7247\u306b\u4ed8\u7740\u672b\u7aef\u304c\u542b\u307e\u308c\u308b\u3002<\/li><\/ul>\n\n\n\n<p><p><a name=\"pKAN\"><\/a><\/p>\n<p><\/p>\n\n&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">pKAN<\/h3>\n\n\n\n<ul class=\"wp-block-list\"><li>4207 \u500b\u306e\u5869\u57fa\u5bfe<\/li><li>\u5358\u4e00\u8907\u88fd\u958b\u59cb\u70b9<\/li><li>\u6297\u751f\u7269\u8cea&nbsp;<b>\u30ab\u30ca\u30de\u30a4\u30b7\u30f3<\/b>&nbsp;kanamycin\uff08 \u30b9\u30c8\u30ec\u30d7\u30c8\u30de\u30a4\u30b7\u30f3\u3068\u540c\u3058\u30a2\u30df\u30ce\u914d\u7cd6\u4f53\u306b\u5c5e\u3059\u308b\u6297\u751f\u7269\u8cea \uff09\u8010\u6027\u306e\u907a\u4f1d\u5b50 (<em>&nbsp;<b>kan<sup>r<\/sup><\/b>&nbsp;<\/em>)<\/li><li><b>BamHI&nbsp;<\/b>\u306b\u3088\u308b\u5358\u7d14\u5207\u65ad<\/li><li><b>HindIII&nbsp;<\/b>\u306b\u3088\u308b\u5358\u7d14\u5207\u65ad<\/li><\/ul>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"\/text\/wp-content\/uploads\/2020\/02\/pAMPpKAN.gif\" alt=\"\"\/><\/figure>\n\n\n\n<p><a name=\"electrophoresis\"><\/a><\/p>\n\n\n\n<p>pKAN \u3092 BamHI \u3068 HindIII \u306e\u4e21\u65b9\u3067\u51e6\u7406\u3059\u308b\u3068\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><b>2332<\/b>&nbsp;\u500b\u306e\u5869\u57fa\u5bfe\u306e\u65ad\u7247<\/li><li><em><b>kan<sup>r<\/sup><\/b>&nbsp;<\/em>\u907a\u4f1d\u5b50\u3092\u542b\u3080&nbsp;<b>1875<\/b>&nbsp;\u500b\u306e\u5869\u57fa\u5bfe\u306e\u65ad\u7247 ( \u305f\u3060\u3057\uff0c\u8907\u88fd\u958b\u59cb\u70b9\u306f\u542b\u307e\u308c\u306a\u3044 )<\/li><li>\u4e21\u65ad\u7247\u306b\u4ed8\u7740\u672b\u7aef\u304c\u542b\u307e\u308c\u308b\u3002<\/li><\/ul>\n\n\n\n<p>\u5236\u9650\u9175\u7d20\u3067\u51e6\u7406\u3057\u305f\u8a66\u6599\u3092\u30a2\u30ac\u30ed\u30fc\u30b9\u30fb\u30b2\u30eb\u306a\u3069\u3067&nbsp;<a href=\"\/text\/?page_id=497#serum\">\u96fb\u6c17\u6cf3\u52d5<\/a>&nbsp;electrophoresis \u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\uff0c\u3053\u308c\u3089\u306e\u65ad\u7247\u3092\u8996\u899a\u7684\u306b\u3068\u3089\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002\u76f4\u6d41\u96fb\u3092\u4ed8\u52a0\u3059\u308b\u3068\uff0c\u8ca0\u306b\u8377\u96fb\u3057\u3066\u3044\u308b&nbsp;<a href=\"\/text\/wp-content\/uploads\/2020\/02\/Phosphate.gif\">\u30ea\u30f3\u9178\u57fa<\/a>&nbsp;\u3092\u3082\u3064\u305f\u3081\u306b\uff0c DNA \u306f\u967d\u96fb\u6975\u65b9\u5411\u3078\u6cf3\u52d5\u3059\u308b\u3002\u5c0f\u3055\u3044\u65ad\u7247\u307b\u3069\uff0c\u30b2\u30eb\u5185\u3092\u65e9\u304f\u79fb\u52d5\u3059\u308b\u3002<\/p>\n\n\n\n<a name=\"Ligation_Possibilities\"><\/a>\n<p><\/p>\n\n&nbsp;\n\n\n\n<h3 class=\"wp-block-heading\">\u3044\u308d\u3044\u308d\u306a\u65ad\u7247\u306e\u518d\u7d50\u5408<\/h3>\n\n\n\n<p>\uff12\u7a2e\u985e\u306e\u5236\u9650\u9175\u7d20\u3092\u53d6\u308a\u9664\u304d\uff0c<a href=\"\/text\/?page_id=713#helicase\">DNA \u30ea\u30ac\u30fc\u30bc<\/a>&nbsp;\u304c\u50cd\u304f\u3088\u3046\u306a\u72b6\u614b\u306b\u3059\u308b\u3068\uff0c\u3053\u308c\u3089\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u65ad\u7247\u306f\u518d\u7d50\u5408\u3057\u3066\u3057\u307e\u3046\u3002<\/p>\n\n\n\n<p>pKAN \u3068 pAMP \u306e\u65ad\u7247\u3092\u6df7\u5408\u3059\u308b\u3068\uff0c\u305d\u308c\u305e\u308c\u306e\u5206\u5b50\u304c\u518d\u7d50\u5408\u3059\u308b\u53ef\u80fd\u6027\u3082\u8907\u6570\u3042\u308b\u3002\u307e\u305f\uff0c\u5834\u5408\u306b\u3088\u3063\u3066\u306f\uff0c\u3064\u307e\u308a2\u500b\u306e\u8907\u88fd\u958b\u59cb\u70b9\u3092\u542b\u3093\u3060\u308a\uff0c1\u500b\u3082\u542b\u307e\u306a\u3044\u5206\u5b50\u306f\u6a5f\u80fd\u7684\u306a\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u623b\u3089\u306a\u3044\u3053\u3068\u3082\u3042\u308b\u3060\u308d\u3046\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"\/text\/wp-content\/uploads\/2020\/02\/RecombinantPlasmid.gif\" alt=\"\"\/><\/figure>\n\n\n\n<p>\u8208\u5473\u6df1\u3044\u53ef\u80fd\u6027\u3068\u3057\u3066\u306f\uff0c<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>3755 \u500b\u306e\u5869\u57fa\u5bfe\u306e pAMP \u65ad\u7247 (&nbsp;<b><em>amp<sup>r<\/sup><\/em><\/b>&nbsp;\u907a\u4f1d\u5b50\u3068\u8907\u88fd\u958b\u59cb\u70b9\u3092\u542b\u3080 ) \u3068<\/li><li>1875 \u500b\u306e\u5869\u57fa\u5bfe\u306e pKAN \u65ad\u7247 (<em>&nbsp;<b>kan<sup>r<\/sup><\/b><\/em>&nbsp;\u907a\u4f1d\u5b50\u3092\u542b\u3080 ) \u3068\u306e\u7d50\u5408\u3067\u3042\u308b\u3002<\/li><\/ul>\n\n\n\n<p><b>DNA \u30ea\u30ac\u30fc\u30bc<\/b>\u3067\u9023\u7d50\u3055\u305b\u308b\u3068\uff0c\u3053\u308c\u3089\u306e\u5206\u5b50\u306f\uff0c\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u3068\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u306e&nbsp;<b>\u4e21\u65b9<\/b>&nbsp;\u306b\u8010\u6027\u3092\u793a\u3059\u6a5f\u80fd\u7684\u306a\u30d7\u30e9\u30b9\u30df\u30c9\uff08 \u306e\u69d8 \uff09\u306b\u306a\u308b\u3002\u3053\u308c\u304c&nbsp;<b>\u7d44\u307f\u63db\u3048 DNA<\/b>&nbsp;\u5206\u5b50\u306e\u4f8b\u3067\u3042\u308b\u3002<\/p>\n\n\n\n<p>\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u3088\u3046\u306b\u6a5f\u80fd\u7684\u306a\u5206\u5b50\u3068\u3057\u3066\u632f\u308b\u821e\u3048\u308b\u306e\u306f\u8907\u88fd\u958b\u59cb\u70b9\u304c\u3042\u308b\u305f\u3081\u3067\u3042\u308b\u304c\uff0c\u3053\u308c\u306f pAMP \u306b\u4f9d\u5b58\u3059\u308b\u3082\u306e\u3067\u3042\u308b\u3002\u3057\u305f\u304c\u3063\u3066\uff0c\u3053\u306e\u5834\u5408 pAMP \u3092&nbsp;<b>\u30d9\u30af\u30bf\u30fc<\/b>&nbsp;\u3068\u547c\u3076\u3002<\/p>\n\n\n\n<p><p><a name=\"Transforming_E._coli\"><\/a><\/p>\n<p><\/p>\n\n&nbsp;<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u5927\u8178\u83cc\u306e\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048<\/h2>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"\/text\/wp-content\/uploads\/2020\/02\/Colonies.gif\" alt=\"\"\/><\/figure>\n\n\n\n<p>\u518d\u7d50\u5408\u3057\u305f\u5206\u5b50\u306e\u6df7\u5408\u6db2\u3067\u5927\u8178\u83cc\u3092\u51e6\u7406\u3059\u308b\u3068\uff0c\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u3068\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u306e\u4e21\u65b9\u304c\u5b58\u5728\u3057\u3066\u3082\u767a\u80b2\u3067\u304d\u308b\u30b3\u30ed\u30cb\u30fc\u304c\u51fa\u73fe\u3059\u308b\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>\u5927\u8178\u83cc\u306e\u6df7\u6fc1\u6db2\u3092\uff0c\u518d\u7d50\u5408\u3057\u305f DNA \u5206\u5b50\u306e\u6df7\u5408\u6db2\u3067\u51e6\u7406\u3059\u308b\u3002<\/li><li>\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u3068\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u3092\u542b\u3080\u5bd2\u5929\u57f9\u5730\u306b\u5927\u8178\u83cc\u6df7\u6fc1\u6db2\u3092\u5857\u5e03\u3059\u308b\u3002<\/li><li>\u7fcc\u65e5\uff0c\u4e21\u65b9\u306e\u6297\u751f\u7269\u8cea\u306b\u8010\u6027\u3092\u793a\u3059\u7d30\u80de\u304c\uff0c\u6570\u5104\u306b\u3082\u9054\u3059\u308b\u5f62\u8cea\u8ee2\u63db\u3057\u305f\u7d30\u80de\u306e\u30b3\u30ed\u30cb\u30fc\u3068\u3057\u3066\u78ba\u8a8d\u3067\u304d\u308b\u307e\u3067\u5897\u6b96\u3059\u308b\u3002<\/li><li>\u305d\u308c\u305e\u308c\u306e\u30b3\u30ed\u30cb\u30fc\u306f\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u7d30\u80de\u306e&nbsp;<b>\u30af\u30ed\u30fc\u30f3<\/b>&nbsp;\u3067\u3042\u308b\u3002<\/li><\/ul>\n\n\n\n<p>\u3057\u304b\u3057\uff0c\u5927\u8178\u83cc\u306f\u8907\u6570\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u540c\u6642\u306b\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048\u3067\u304d\u308b\u306e\u3067\uff0c\u5f62\u8cea\u8ee2\u63db\u3057\u305f\u7d30\u80de\u304c\u7d44\u307f\u63db\u3048\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u3082\u3063\u3066\u3044\u308b\u304b\u78ba\u8a8d\u3057\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u3002<\/p>\n\n\n\n<p>\u4e8c\u91cd\u306e\u8010\u6027\u3092\u793a\u3059\u30b3\u30ed\u30cb\u30fc ( \u30af\u30ed\u30fc\u30f3 ) \u306eDNA \u3092\u96fb\u6c17\u6cf3\u52d5\u3059\u308b\u3068\uff0c\u3044\u308d\u3044\u308d\u306a\u3053\u3068\u304c\u5206\u304b\u308b\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li><b>\u7d44\u307f\u63db\u3048\u30d7\u30e9\u30b9\u30df\u30c9<\/b>&nbsp;\u3060\u3051\u304b\u3089\u8010\u6027\u3092\u7372\u5f97\u3057\u305f\u7d30\u80de\u304b\u3089\u306e\u30d7\u30e9\u30b9\u30df\u30c9 DNA \u306f&nbsp;<b>3755<\/b>-bp \u3068&nbsp;<b>1875<\/b>-bp \u306e\u30d0\u30f3\u30c9\u306e\u307f\u3092\u793a\u3059 (&nbsp;<b>Clone 1<\/b>, lane 3 ) \u3002<figure><img loading=\"lazy\" decoding=\"async\" src=\"\/text\/wp-content\/uploads\/2020\/02\/ThreeClones.gif\" alt=\"\" width=\"357\" height=\"225\" align=\"right\"><\/figure><\/li><li><b>Clone 2<\/b>&nbsp;( Lane 4 ) \u306f\u518d\u7d50\u5408\u3057\u305f pAMP \u3068 pKAN \u306b\u3088\u3063\u3066\u540c\u6642\u306b\u907a\u4f1d\u5b50\u304c\u7d44\u307f\u63db\u3048\u3089\u308c\u305f\u3002( \u3053\u306e\u5834\u5408\uff0c\u7d44\u307f\u63db\u3048\u5206\u5b50\u3082\u53d7\u3051\u7d99\u3044\u3067\u3044\u308b\u304b\u3069\u3046\u304b\u306f\u65ad\u5b9a\u3067\u304d\u306a\u3044 \uff09<\/li><li><b>Clone 3<\/b>&nbsp;( Lane 5 ) \u306f pKAN \u3068\u540c\u69d8\u306b\u7d44\u307f\u63db\u3048\u5206\u5b50\u306b\u3088\u3063\u3066\u3082\u5f62\u8cea\u8ee2\u63db\u3055\u308c\u3066\u3044\u308b\u3002<\/li><\/ul>\n\n\n\n<p><p><a name=\"Cloning_other_Genes\"><\/a><\/p>\n<p><\/p>\n\n&nbsp;<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u907a\u4f1d\u5b50\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0<\/h2>\n\n\n\n<p>\u4e0a\u8ff0\u3057\u305f\u7d44\u307f\u63db\u3048\u30d9\u30af\u30bf\u30fc\u306f\u305d\u308c\u81ea\u8eab\u4ed6\u306e\u907a\u4f1d\u5b50\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u305f\u3081\u306e\u30c4\u30fc\u30eb\u3068\u3057\u3066\u6709\u7528\u3067\u3042\u308b\u3002 \u4eca\uff0c\u305d\u306e&nbsp;<b>\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50<\/b>&nbsp;(&nbsp;<b>kan<sup>r<\/sup><\/b>&nbsp;) \u5185\u306b\u6b21\u306b\u5358\u4e00\u914d\u5217\u304c\u3042\u3063\u305f\u3068\u4eee\u5b9a\u3057\u3088\u3046\u3002<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\"><p>5&#8242; GAATTC 3&#8242;<br>3&#8242; CTTAAG 5&#8242;<\/p><\/blockquote>\n\n\n\n<p>\u3053\u308c\u306f\uff0c\u5236\u9650\u9175\u7d20&nbsp;<b><a href=\"\/text\/?page_id=1050\">EcoRI<\/a><\/b>&nbsp;\u3067\u5207\u65ad\u3067\u304d\uff0c\u4ed8\u7740\u7aef\u3092\u3064\u304f\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002<\/p>\n\n\n\n<p>\u3053\u3053\u3067\uff0c\u305f\u3068\u3048\u3070\u30d2\u30c8\u7d30\u80de\u306e\u4ed6\u306e DNA \u8a66\u6599\u3092 EcoRI \u3067\u51e6\u7406\u3057\uff0c\u540c\u69d8\u306e\u4ed8\u7740\u7aef\u304c\u5f62\u6210\u3055\u308c\u305f\u3068\u3057\u3088\u3046\u3002EcoRI \u51e6\u7406\u30d7\u30e9\u30b9\u30df\u30c9\u3068 DNA \u30ea\u30ac\u30fc\u30bc\u3092\u6df7\u5408\u3059\u308b\u3068\uff0c\u5c11\u6570\u306a\u304c\u3089\u30d2\u30c8\u306e\u5206\u5b50\u304c\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u5c0e\u5165\u3055\u308c\uff0c\u3053\u308c\u304c\u5927\u8178\u83cc\u3092\u5f62\u8cea\u8ee2\u63db\u3059\u308b\u306e\u306b\u5229\u7528\u3067\u304d\u308b\u3002<\/p>\n\n\n\n<p>\u3057\u304b\u3057\uff0c\u30d2\u30c8\u306e DNA \u3092\u3082\u3064\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u3088\u3063\u3066\u5f62\u8cea\u8ee2\u63db\u3055\u308c\u305f\u3053\u308c\u3089\u306e\u5927\u8178\u83cc\u306e\u30af\u30ed\u30fc\u30f3\u306f\u3069\u306e\u3088\u3046\u306b\u691c\u51fa\u3067\u304d\u308b\u306e\u3060\u308d\u3046\u304b\uff1f<\/p>\n\n\n\n<p>\u305d\u306e\u9375\u306f\uff0c&nbsp;<b>kan<sup>r<\/sup><\/b>&nbsp;\u907a\u4f1d\u5b50<b>\u5185<\/b>\u306b\u3042\u308b EcoRI \u9818\u57df\u3067\u3042\u308b\u3002\u3057\u305f\u304c\u3063\u3066\uff0c\u30d2\u30c8\u306e DNA \u304c\u305d\u3053\u306b\u633f\u5165\u3055\u308c\u308b\u3068\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u306f\u7834\u58ca\u3055\u308c\u308b\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"\/text\/wp-content\/uploads\/2020\/02\/ScreeningClones.gif\" alt=\"\"\/><\/figure>\n\n\n\n<p>\u30d9\u30af\u30bf\u30fc\u306b\u3088\u3063\u3066\u5f62\u8cea\u8ee2\u63db\u3055\u308c\u305f\u3059\u3079\u3066\u306e\u5927\u8178\u83cc\u306f\uff0c\u30d2\u30c8DNA \u3092\u3082\u3064\u304b\u3082\u305f\u306a\u3044\u304b\u306b\u304b\u304b\u308f\u3089\u305a\uff0c\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u306e\u5b58\u5728\u4e0b\u3067\u5897\u6b96\u3067\u304d\u308b\u3002\u3057\u304b\u3057\uff0c\u30d2\u30c8 DNA \u3092\u3082\u3064\u30d7\u30e9\u30b9\u30df\u30c9\u306b\u3088\u3063\u3066\u5f62\u8cea\u8ee2\u63db\u3055\u308c\u305f\u5927\u8178\u83cc\u306f\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u306e\u5b58\u5728\u4e0b\u3067\u306f\u5897\u6b96\u3067\u304d\u306a\u3044\u3002<\/p>\n\n\n\n<p>\u3057\u305f\u304c\u3063\u3066\uff0c<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u3060\u3051\u3092\u542b\u3080\u5bd2\u5929\u306b\u51e6\u7406\u3057\u305f\u5927\u8178\u83cc\u306e\u6df7\u6fc1\u6db2\u3092\u5857\u5e03\u3059\u308b\u3002<\/li><li>\u4e00\u6669\u5897\u6b96\u3055\u305b\u308b\u3002<\/li><li>\u660e\u77ad\u306a\u30b9\u30dd\u30c3\u30c8\u3068\u3057\u3066\u540c\u5b9a\u3067\u304d\u308b\u30b3\u30ed\u30cb\u30fc\u3092\u6ec5\u83cc\u3057\u305f\u722a\u694a\u679d\u3067\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u3092\u542b\u3080\u5bd2\u5929\u306b\u79fb\u3057\u66ff\u3048\u308b\u3002<\/li><li>( \u5225\u306e\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u5bd2\u5929\u306b\u3082\u540c\u69d8\u306b\u3059\u308b\u3002)<\/li><li>\u4e00\u6669\u57f9\u990a\u3059\u308b\u3002<\/li><\/ul>\n\n\n\n<p>\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u3067\u5897\u6b96\u3059\u308b\u304c\uff0c\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u3067\u5897\u6b96\u3067\u304d\u306a\u3044\u3053\u308c\u3089\u306e\u30af\u30ed\u30fc\u30f3\u304c\u3072\u3068DNA \u306b\u3088\u3063\u3066\u5f62\u8cea\u8ee2\u63db\u3055\u308c\u305f\u3082\u306e\u3067\u3042\u308b\u3002 ( \u3053\u3053\u3067\u306f\uff0c&nbsp;<b>clones 2<\/b>,&nbsp;<b>5<\/b>, \u306a\u3089\u3073\u306b&nbsp;<b>8<\/b>&nbsp;)<\/p>\n\n\n\n<p><p><a name=\"Some_recombinant_DNA_products_being_used_in_human_therapy\"><\/a><\/p>\n<p><\/p>\n\n&nbsp;<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u6cbb\u7642\u306b\u7528\u3044\u3089\u308c\u308b\u7d44\u307f\u63db\u3048 DNA \u7523\u7269<\/h2>\n\n\n\n<p>\u3053\u306e\u3088\u3046\u306a\u624b\u6cd5\u3092\u7528\u3044\u3066\uff0c\u591a\u304f\u306e\u30d2\u30c8\u907a\u4f1d\u5b50\u304c\u5927\u8178\u83cc\u3084\u9175\u6bcd\u3067\u30af\u30ed\u30fc\u30f3\u304c\u3064\u304f\u3089\u308c\u3066\u3044\u308b\u3002\u3053\u308c\u306f\u30d2\u30c8\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f53\u5916\u3067\u9650\u308a\u306a\u304f\u751f\u7523\u3067\u304d\u308b\u3053\u3068\u3092\u610f\u5473\u3059\u308b\u3002\u30d2\u30c8\u306e\u907a\u4f1d\u5b50\u3092\u7d44\u307f\u8fbc\u307e\u308c\u305f\u7d30\u80de ( \u5927\u8178\u83cc\uff0c\u9175\u6bcd\uff0c\u54fa\u4e73\u52d5\u7269\u7d30\u80de ) \u3092\u57f9\u990a\u3059\u308b\u3053\u3068\u306b\u3088\u3063\u3066\uff0c\u4ee5\u4e0b\u306e\u7269\u8cea\u306e\u751f\u7523\u304c\u884c\u308f\u308c\u3066\u3044\u308b\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>\u7cd6\u5c3f\u75c5\u60a3\u8005\u306e\u305f\u3081\u306e<b><a href=\"\/text\/?page_id=297#Insulin\">\u30a4\u30f3\u30b9\u30ea\u30f3<\/a><\/b><\/li><li>\u8840\u53cb\u75c5 A \u306e\u7537\u6027\u306e\u305f\u3081\u306e\u8840\u6db2\u51dd\u56fa\u00a0<b>\u7b2c VIII \u56e0\u5b50<\/b><\/li><li>\u8840\u53cb\u75c5 B \u306e\u60a3\u8005\u306e\u305f\u3081\u306e\u8840\u6db2\u51dd\u56fa\u00a0<b>\u7b2c IX \u56e0\u5b50<\/b><\/li><li><b>\u30d2\u30c8\u6210\u9577\u30db\u30eb\u30e2\u30f3<\/b>\u00a0(\u00a0<b>GH<\/b>\u00a0)<\/li><li>\u8ca7\u8840\u306e\u51e6\u7406\u306e\u305f\u3081\u306e\u00a0<b>\u30a8\u30ea\u30b9\u30ed\u30dd\u30a8\u30c1\u30f3<\/b>\u00a0erythropoietin ( \u8d64\u8840\u7403\u751f\u6210\u4fc3\u9032\u56e0\u5b50 \uff09<\/li><li>\u6570\u7a2e\u985e\u306e\u00a0<b>\u30a4\u30f3\u30bf\u30fc\u30d5\u30a7\u30ed\u30f3<\/b><\/li><li>\u6570\u7a2e\u985e\u306e\u00a0<b>\u30a4\u30f3\u30bf\u30fc\u30ed\u30a4\u30ad\u30f3<\/b><\/li><li>\u9aa8\u9ac4\u79fb\u690d\u5f8c\u306e\u9aa8\u9ac4\u306e\u523a\u6fc0\u306e\u305f\u3081\u306e\u00a0<b>\u9846\u7c92\u7403\u30de\u30af\u30ed\u30d5\u30a1\u30fc\u30b8\u30b3\u30ed\u30cb\u30fc\u523a\u6fc0\u56e0\u5b50<\/b>\u00a0(\u00a0<b>GM-CSF<\/b>\u00a0)<\/li><li>\uff08\u4f8b\u3048\u3070\u3001\u5316\u5b66\u7642\u6cd5\u306e\u5f8c\u306b\uff09\u597d\u4e2d\u7403\u306e\u751f\u7523\u3092\u523a\u6fc0\u3057\u3066\uff0c\u8840\u4e2d\u306b\u9aa8\u9ac4\u304b\u3089<strong>\u9020\u8840\u5e79\u7d30\u80de<\/strong>\u3092\u52d5\u54e1\u3059\u308b\u305f\u3081\u306e<strong>\u9846\u7c92\u767d\u8840\u7403\u30b3\u30ed\u30cb\u30fc\u523a\u6fc0\u56e0\u5b50<\/strong>\uff08<strong>G-CSF<\/strong>\uff09<\/li><li>\u8840\u6813\u3092\u6eb6\u89e3\u3059\u308b\u00a0<b>\u7d44\u7e54\u30d7\u30e9\u30b9\u30df\u30ce\u30b2\u30f3\u6d3b\u6027\u5316\u56e0\u5b50<\/b>\u00a0(<b>TPA<\/b>)<\/li><li><b>\u91cd\u75c7\u8907\u5408\u578b\u514d\u75ab\u4e0d\u5168<\/b>\u00a0(\u00a0<b>SCID<\/b>\u00a0) \u6cbb\u7642\u7528\u306e\u00a0<b>\u30a2\u30c7\u30ce\u30b7\u30f3\u30fb\u30c7\u30a2\u30df\u30ca\u30fc\u30bc<\/b>\u00a0(\u00a0<b>ADA<\/b>\u00a0)<\/li><li><strong>\u526f\u7532\u72b6\u817a\u30db\u30eb\u30e2\u30f3<\/strong><\/li><li>\u591a\u304f\u306e<strong>\u30e2\u30ce\u30af\u30ed\u30fc\u30ca\u30eb\u6297\u4f53<\/strong><\/li><li>B\u578b\u809d\u708e\u30a6\u30a4\u30eb\u30b9\u306b\u5bfe\u3057\u3066\u4e88\u9632\u63a5\u7a2e\u3092\u3059\u308b\u305f\u3081\u306e<strong>B\u578b\u809d\u708e\u8868\u9762\u6297\u539f<\/strong>\uff08HBsAg\uff09<\/li><li>\u907a\u4f1d\u6027\u8840\u7ba1\u6d6e\u816b\u3092\u6cbb\u7642\u3059\u308b\u306e\u306b\u7528\u3044\u3089\u308c\u308b<strong>C1\u30a4\u30f3\u30d2\u30d3\u30bf\u30fc<\/strong>\uff08C1INH\uff09<\/li><\/ul>\n\n\n\n<p>\u3055\u3089\u306b\u591a\u304f\u306e\u4f8b\u304c\u958b\u767a\u4e2d\u3067\u3042\u308b\u3002\u3000<\/p>\n\n\n\n<hr class=\"wp-block-separator is-style-wide\"\/>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"\"><tbody><tr><td><a href=\"\/text\/?page_id=1022\">\u6700\u521d\u306b\u623b\u308b<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<hr class=\"wp-block-separator is-style-wide\"\/>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"\"><tbody><tr><td><b>\u30e1\u30cb\u30e5\u30fc\u306e\u30da\u30fc\u30b8\u3078\u623b\u308b<\/b><\/td><\/tr><tr><td>\n<ul>\n<li><a href=\"\/text\/#Reproduction\">\u5bb6\u755c\u7e41\u6b96\u5b66<\/a><\/li>\n<li><a href=\"\/text\/#Breeding_and_Genetics\">\u5bb6\u755c\u80b2\u7a2e\u5b66<\/a><\/li>\n<li><a href=\"\/text\/#References\">\u53c2\u8003\u6587\u732e<\/a><\/li>\n<\/ul>\n<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>March 22, 2020<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7d44\u307f\u63db\u3048\u578b DNA \u3068\u907a\u4f1d\u5b50\u30af\u30ed\u30fc\u30cb\u30f3\u30b0 25 April 2014 \u7248\u3092\u7ffb\u8a33\u4e00\u90e8\u6539\u5909 \u3053\u306e\u30da\u30fc\u30b8\u306e\u5185\u5bb9 \u6982\u8981\u56f3 \u30d7\u30e9\u30b9\u30df\u30c9 \u4e3b\u306a\u4f8b pAMP pKAN \u3044\u308d\u3044\u308d\u306a\u65ad\u7247\u306e\u518d\u7d50\u5408 \u5927\u8178\u83cc\u306e\u907a\u4f1d\u5b50\u7d44\u307f\u63db\u3048 \u907a\u4f1d\u5b50\u306e\u30af\u30ed\u30fc\u30cb\u30f3 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":183,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-1022","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/marimo3take.wafflecell.com\/text\/index.php?rest_route=\/wp\/v2\/pages\/1022","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/marimo3take.wafflecell.com\/text\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/marimo3take.wafflecell.com\/text\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/marimo3take.wafflecell.com\/text\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/marimo3take.wafflecell.com\/text\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1022"}],"version-history":[{"count":39,"href":"https:\/\/marimo3take.wafflecell.com\/text\/index.php?rest_route=\/wp\/v2\/pages\/1022\/revisions"}],"predecessor-version":[{"id":4187,"href":"https:\/\/marimo3take.wafflecell.com\/text\/index.php?rest_route=\/wp\/v2\/pages\/1022\/revisions\/4187"}],"up":[{"embeddable":true,"href":"https:\/\/marimo3take.wafflecell.com\/text\/index.php?rest_route=\/wp\/v2\/pages\/183"}],"wp:attachment":[{"href":"https:\/\/marimo3take.wafflecell.com\/text\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1022"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}